A biochemical and proteomic approach to the analysis of tomato mutant fruit growth

To assess the effects of ABA deficiency on tomato fruit growth, mutant flacca was grown in an optimal soil water regime and various analyzes were performed, including morphological (fruit number, diameter biomass), physiological (duration growth rate), biochemical (ABA accumulation, enzyme cell wall peroxidase activity) as well proteomics. The analysis showed that slower rate development resulted smaller fruits comparison to wild-type fruits. temporal dynamics activity content our experiment indicated opposite relationship during development. Proteomic down-regulation most proteins from carbon amino acid metabolism, translation processing proteins, energy metabolism wall-related compared wild type, reduced metabolic flux which reflected a size mutant. These findings also limited sources, could be responsible for ABA-deficient up-regulation sulfur oxygen-evolving enhancer implicated maintenance photosynthesis late expansion phase, slows down transition ripening stage. majority antioxidative stress defence down-regulated fruits, related role different enzymes regulating cessation growth.